Journal: Malaria Journal

Article Title: Reduced ADAMTS13 activity is associated with an ADAMTS13 SNP, fever and microparticles in a malaria-like model

doi: 10.1186/1475-2875-13-3

Figure Lengend Snippet: Genotypic association of rs4962153 genotype and ADAMTS13 antigen and activity. (A) The mean of ADAMTS13 antigen levels in participants with AA, AG and GG genotype of rs4962153 were 683.91, 568.02 and 327.26 ng/ml, respectively. ADAMTS13 antigen levels in participants with AA genotype were significantly reduced compared to both of AG and GG genotype ( P < 0.05 and < 0.001, respectively). Furthermore, ADAMTS13 antigen in participants with AG genotype was also significantly decreased when compared to GG genotype ( P < 0.05). (B) ADAMTS13 activity levels in participants with AA genotype (mean = 58.26%), were significantly reduced when compared to AG genotype (mean = 84.78%; P < 0.05) and GG genotype (mean = 95.53%; P < 0.001). The middle line referred mean of each individual genotype. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Citrated plasma samples were collected from those participants and then the level of ADAMTS13 antigen and activity were performed using commercial kits (Quantikine Human ADAMTS13 ELISA kit; R&D Systems Inc., U.S.A, and Technozyme ADAMTS13 activity ELISA; Technoclone, Austria).

Techniques: Activity Assay

Journal: Malaria Journal

Article Title: Reduced ADAMTS13 activity is associated with an ADAMTS13 SNP, fever and microparticles in a malaria-like model

doi: 10.1186/1475-2875-13-3

Figure Lengend Snippet: Inhibitory effect of MPs on ADAMTS13 activity. Non-filtered plasma samples presented ADAMTS13 activity 86.06%, which was significant lower than the filtered-plasma samples (mean = 104.03%). * P < 0.05.

Article Snippet: Citrated plasma samples were collected from those participants and then the level of ADAMTS13 antigen and activity were performed using commercial kits (Quantikine Human ADAMTS13 ELISA kit; R&D Systems Inc., U.S.A, and Technozyme ADAMTS13 activity ELISA; Technoclone, Austria).

Techniques: Activity Assay

Journal: Malaria Journal

Article Title: Reduced ADAMTS13 activity is associated with an ADAMTS13 SNP, fever and microparticles in a malaria-like model

doi: 10.1186/1475-2875-13-3

Figure Lengend Snippet: Effects of Febrile temperatures and Incubation time to ADAMTS13 activity*

Article Snippet: Citrated plasma samples were collected from those participants and then the level of ADAMTS13 antigen and activity were performed using commercial kits (Quantikine Human ADAMTS13 ELISA kit; R&D Systems Inc., U.S.A, and Technozyme ADAMTS13 activity ELISA; Technoclone, Austria).

Techniques: Incubation, Activity Assay

Journal: Malaria Journal

Article Title: Reduced ADAMTS13 activity is associated with an ADAMTS13 SNP, fever and microparticles in a malaria-like model

doi: 10.1186/1475-2875-13-3

Figure Lengend Snippet: ADAMTS13 activity was reduced in the incubation time and temperature-dependent manners. (A, B &C) Clear evidence of a time-dependent inhitition was observed either at 37°C, 38°C, or 39°C. * P < 0.05, ** P < 0.01, *** P < 0.001. (D) A temperature-dependent inhibition was observed. ADAMTS13 activity was significantly decreased at 38°C and 39°C incubation temperature, compared to 37°C for each incubation time except for 24 hr of 38°C compared to 37°C (NS = Not significant). All results represent mean ± SEM.

Article Snippet: Citrated plasma samples were collected from those participants and then the level of ADAMTS13 antigen and activity were performed using commercial kits (Quantikine Human ADAMTS13 ELISA kit; R&D Systems Inc., U.S.A, and Technozyme ADAMTS13 activity ELISA; Technoclone, Austria).

Techniques: Activity Assay, Incubation, Inhibition

Journal: Malaria Journal

Article Title: Reduced ADAMTS13 activity is associated with an ADAMTS13 SNP, fever and microparticles in a malaria-like model

doi: 10.1186/1475-2875-13-3

Figure Lengend Snippet: Level of ADAMTS13 activity in plasma incubated in normal and febrile temperature

Article Snippet: Citrated plasma samples were collected from those participants and then the level of ADAMTS13 antigen and activity were performed using commercial kits (Quantikine Human ADAMTS13 ELISA kit; R&D Systems Inc., U.S.A, and Technozyme ADAMTS13 activity ELISA; Technoclone, Austria).

Techniques: Activity Assay, Incubation

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Cathepsin K deficiency prevented stress-related thrombosis in a mouse FeCl 3 model

doi: 10.1007/s00018-024-05240-0

Figure Lengend Snippet: CTSK knockout lowered the artery endothelial damage and FeCl 3 -induced thrombosis in the stressed mice. A , B Representative images and quantitative data depicting the thrombus area [hematoxylin and eosin (H&E) staining: left panels ] and the numbers of CD31 + cells (immunostaining: left middle panels ) and endothelial cells of the thrombotic lesions (TEM: right middle panels ) in the two groups ( n = 5–7). The representative immunofluorescence shows CD31 + cells in both experimental groups ( right panels ). C The quantitative data of the weight and length of the thrombi in CTSK + / + and CTSK −/− mice after 2 weeks of immobilized stress ( n = 9). D Body weights of the two groups on days 0 and 21 ( n = 8). E ELISA results for the levels of blood PAI-1, MCP-1, VCAM-1, IL-18, ADAMTS13, and vWF on day 21 of the stress ( n = 7). Results are mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, N.S. (not significant) vs. the stressed CTSS + / + group by one-way ANOVA and Tukey’s post hoc test or Student’s t- test. Scale bars 2 μm for the TEM and 75, 100 and 500 μm for the others

Article Snippet: The enzyme-linked immunosorbent assay (ELISA) kits for the vascular cell adhesion molecule-1 (VCAM-1; MVC00) was from R&D Systems (Minneapolis, MN), and ADAMTS13 (cat. no. CSB-EL001301MO) was purchased from Cusabio (Houston, TX).

Techniques: Knock-Out, Staining, Immunostaining, Immunofluorescence, Enzyme-linked Immunosorbent Assay